Use of doubled haploid technology for development of stable drought tolerant bread wheat (Triticum aestivum L.) transgenics

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Use of doubled haploid technology for development of stable drought tolerant bread wheat (Triticum aestivum L.) transgenics.

Anther culture-derived haploid embryos were used as explants for Agrobacterium-mediated genetic transformation of bread wheat (Triticum aestivum L. cv CPAN1676) using barley HVA1 gene for drought tolerance. Regenerated plantlets were checked for transgene integration in T₀ generation, and positive transgenic haploid plants were doubled by colchicine treatment. Stable transgenic doubled haploid ...

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ژورنال

عنوان ژورنال: Plant Biotechnology Journal

سال: 2010

ISSN: 1467-7644

DOI: 10.1111/j.1467-7652.2010.00561.x